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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
S100钙结合蛋白A10(S100A10),也称为p11,是由S100A10基因和基因编码的蛋白质。它是包含两个EF-hand钙结合基序的S100家族蛋白质的成员。S100蛋白位于多种细胞的细胞质和/或细胞核中。它们调节许多细胞过程,如细胞周期进程和分化。它与神经递质的转运有关。它存在于人类和其他哺乳动物的大脑中,与情绪调节有关。此外,由于它与血清素信号蛋白的相互作用及其与情绪障碍症状的相关性,它是药物治疗的新潜在靶点。它是细胞结构支架的一个组成部分,通过其与膜联蛋白II的结合与质膜蛋白相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠S100钙结合蛋白A10(S100A10)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠S100钙结合蛋白A10(S100A10)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat S100A10. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat S100A10 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.