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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Toll样受体4(TLR4),是由基因TLR4编码的蛋白质,是一种膜蛋白质,也被称为CD284,是Toll样受体家族的成员,属于模式识别受体(PRR)家族。它的激活导致细胞内信号通路NF-κB和炎症细胞因子的产生,后者负责激活先天免疫系统。表达TRL4的细胞是骨髓(如红细胞、粒细胞、巨噬细胞)而不是淋巴细胞(如T细胞、B细胞、NK细胞)。大多数骨髓细胞还表达高水平的CD14,这有助于LPS激活TLR4。它最著名的是识别脂多糖 (LPS),脂多糖是许多革兰氏阴性细菌(例如奈瑟菌属)和某些革兰氏阳性菌中存在的一种成分。它的配体还包括几种病毒蛋白、多糖和多种内源性蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠Toll样受体4(TLR4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠Toll样受体4(TLR4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat TLR4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat TLR4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.