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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α1-微球蛋白(α1-MG)是一种小球状蛋白。它分布在血浆和所有器官的血管外组织中。它在身体的大多数细胞中合成,但主要是在肝脏。它结合并降解血红素,是一种自由基清除剂,也是一种还原酶。有人提出了一个模型,其中它被描述为一个循环的 "垃圾箱",不断地从组织中清除自由基和氧化剂,特别是血红素。它随后被运送到肾脏,在那里被分解。因此,该蛋白被认为能保护细胞和组织免受异常高浓度的自由血红蛋白和/或活性氧(也称为 "氧化应激")的损害。它还具有免疫调节作用:淋巴细胞和中性粒细胞的免疫反应部分受到它的抑制。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠α1微球蛋白(α1-MG)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠α1微球蛋白(α1-MG)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat α1-MG. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat α1-MG present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.