计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
三叶因子1,也被称为TFF1,三叶因子1是一种蛋白质,由TFF1基因(也称为PS2基因)编码。它是三叶草家族的成员,这个家族成员的特征是至少有一个三叶草基序的副本,这是一个包含三个保守二硫化的40氨基酸结构域。它们是在胃肠粘膜中表达的稳定分泌蛋白。它们的功能尚未定义,但它们可以保护粘膜免受侮辱,稳定粘液层并影响上皮的愈合。该基因和另外两个相关的三叶草家族成员基因在21号染色体上的一个簇中被发现。它的表达在胃癌中经常丢失,可能是通过DNA甲基化的机制,因此被认为是肿瘤抑制基因。它主要在胃粘膜的中央凹上皮表面细胞中表达,并在炎症期间表现出增加表达的模式。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠三叶因子1(TFF1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠三叶因子1(TFF1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat TFF1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat TFF1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.