计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Endocan,以前称为内皮细胞特异性分子-1,由 ESM1 基因编码,是一种50kDa 的可溶性蛋白多糖,由 165 个氨基酸的成熟多肽和一条与 137 位丝氨酸残基共价连接的硫酸真皮链构成。该基因的表达受细胞因子的调控,表明它可能在内皮细胞依赖的病理疾病中发挥作用。研究表明,在血管内皮生长因子(VEGF)或成纤维细胞生长因子 2(FGF-2)等促血管生成的生长因子存在时,Endocan 的表达会增加。在高血管化的癌症中,通过使用针对endocan/ESM-1 的单克隆抗体进行免疫组化,检测出 endocan 的过度表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠内皮细胞特异性分子(Endocan;ESM-1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠内皮细胞特异性分子(Endocan;ESM-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat Endocan;ESM-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat Endocan;ESM-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.