BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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分泌型卷曲相关蛋白2是由SFRP2基因编码的蛋白。该基因编码的SFRP家族成员含有一个与Frizzled蛋白的推定Wnt结合位点相同的富半胱氨酸结构域。SFRPs作为Wnt信号传导的可溶性调节剂。该基因的甲基化是存在结肠直肠癌的一个潜在标志。SFRP2基因在来自子宫颈癌前病变的人乳头瘤病毒阳性角质细胞中被检测到逐步过度表达，处于不同的恶性程度。因此，该基因可能与肿瘤发生有关，可能是子宫颈癌前病变进展的潜在预后标志物。与SFRP2相关的疾病包括食道基底层鳞状细胞癌和Synpolydactyly。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠分泌型卷曲相关蛋白2(SFRP2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠分泌型卷曲相关蛋白2(SFRP2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat SFRP2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat SFRP2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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