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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
前蛋白转化酶枯草溶菌素1(PCSK1),也被称为激素原转化酶、激素原转化酶3或神经内分泌转化酶1,通常缩写为PC1或PC3,是由PCSK1基因编码的酶。它和PCSK2差异性地切割前啡肽原,它们共同作用于胰岛中的胰岛素原和胰高血糖素原。它只存在于神经内分泌细胞中,如脑、垂体和肾上腺细胞,并且最常在激素原内的一对碱性残基后裂解,但偶尔也会在单一精氨酸后裂解。它与一种称为proSAAS的蛋白质结合,该蛋白质也代表其内源性抑制剂。它是钙活化的丝氨酸内蛋白酶(意味着丝氨酸残基是水解底物内肽键的活性位点的一部分)。它与称为枯草杆菌蛋白酶的细菌酶有关。它将前肾上腺素转化为肾素。该基因的变异可能与肥胖有关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠前蛋白转化酶枯草溶菌素1(PCSK1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠前蛋白转化酶枯草溶菌素1(PCSK1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat PCSK1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat PCSK1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.