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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
基质金属蛋白酶12(MP-12),也称为巨噬细胞金属弹性蛋白酶或巨噬细胞弹性蛋白酶,是由MMP12基因编码的酶,该基因是11号染色体上MMP基因簇的一部分。它会降解弹性蛋白,并在巨噬细胞和其他基质细胞中高度表达。它对巨噬细胞迁移至关重要,也存在于肥大软骨细胞和破骨细胞中。与它相关的疾病包括肺气肿和疱疹样皮炎。其相关途径包括结直肠癌转移和细胞外基质降解。它可能在动脉瘤形成中发挥作用,也在肺气肿的发展中起作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠基质金属蛋白酶12(MMP-12)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠基质金属蛋白酶12(MMP-12)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat MMP-12. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat MMP-12 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.