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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
基质金属蛋白酶3(MMP-3),也称为Stromelysin-1,是由MMP3基因编码的酶。该基因是位于染色体11q22.3的MMP基因簇的一部分。它的估计分子量为54 kDa。它降解II型、III型、IV型、IX型和X型胶原蛋白、蛋白聚糖、纤连蛋白、层粘连蛋白和弹性蛋白。此外,它还可以激活其他MMP,如MMP-1、MMP-7和MMP-9,使MMP-3在结缔组织重塑中至关重要。它也被认为参与伤口修复,动脉粥样硬化的进展和肿瘤的发生。除了在细胞外空间的经典作用外,它还可以进入细胞核并控制转录,本身可以进入细胞核并调节CTGF / CCN2基因等靶基因。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠基质金属蛋白酶3(MMP-3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠基质金属蛋白酶3(MMP-3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat MMP-3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat MMP-3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.