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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
组织金属蛋白酶抑制因子2(TIMP2),是一种基因和相应的蛋白质。该基因是TIMP基因家族的成员。该蛋白质被认为是转移抑制因子。它是MMP-2的特异性抑制剂,其可与活化的或无活性MMP-2结合,终止MMP-2的活动,有效的抑制MMP-2的胶原及明胶分解活性,抑制癌细胞转移及侵袭。许多研究表明,它和TIMP-2是2个与肿瘤侵袭性有关的指标,在细胞外基质合成及降解代谢平衡中起着至关重要的作用。肿瘤中MMP-2和TIMP-2之间平衡的打破是肿瘤进展的一个重要因素之一。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠基质金属蛋白酶抑制因子2(TIMP-2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠基质金属蛋白酶抑制因子2(TIMP-2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat TIMP-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat TIMP-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.