计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
多巴胺转运蛋白(也称多巴胺活性转运体,DAT,SLC6A3)是一种跨膜蛋白,它将神经递质多巴胺从突触裂隙中泵出,回到细胞膜。在细胞膜中,其他转运体将多巴胺封存到囊泡中,以便储存和随后释放。通过DAT的多巴胺再摄取提供了多巴胺从突触中被清除的主要机制。编码DAT蛋白的基因位于5号染色体上,由15个编码外显子组成,长度大约为64kbp。DAT和多巴胺相关疾病之间的关联证据来自于DAT基因(DAT1)中的一种遗传多态性,即所谓的VNTR,它影响了蛋白质的表达量。DAT是一种完整的膜蛋白,它将多巴胺从突触裂隙中移除并沉积到周围的细胞中,从而终止了神经递质的信号。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠多巴胺转运蛋白(DAT)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠多巴胺转运蛋白(DAT)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat DAT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat DAT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.