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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
离子化钙结合适配分子 1(Iba-1)也被称为异体器官炎症因子 1(AIF-1), 是一种蛋白质,由 AIF1 基因编码。AIF1 基因位于主要组织相容性复合体 III 类区域的一个片段内。该基因在睾丸、脾脏和大脑中高度表达,但在肺和肾 脏中表达较弱。在脑细胞中,Iba-1 基因在小胶质细胞中强烈而特异地表达。 循环中的巨噬细胞也表达 Iba-1。AIF1 是一种存在于细胞质中的蛋白质,它 在进化上是高度保守的。它也可能与其他三种蛋白相同,即 Iba-2、MRF-1 (小胶质细胞反应因子)和 daintain。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠小胶质细胞标志物(Iba-1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠小胶质细胞标志物(Iba-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat Iba-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat Iba-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.