计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
干扰素诱导的 T 细胞趋化因子(ITAC)也被称为 C-X-C 动点趋化因子 11 (CXCL11)和干扰素-γ诱导的蛋白 9(IP-9),是一种蛋白质,由 CXCL11 基因编码。C-X-C 动点趋化因子 11 是属于 CXC 趋化因子家族的一种小型细 胞因子。它在外周血白细胞、胰腺和肝脏中高度表达,在胸腺、脾脏和肺中适度表达,在小肠、胎盘和前列腺中表达水平较低。CXCL11 的基因表达被 IFN-γ和 IFN-β强烈诱导,被 IFN-α弱诱导。这种趋化因子通过与细胞表面的 趋化因子受体 CXCR3 相互作用而对其靶细胞产生影响,其亲和力高于该受 体的其他配体 CXCL9 和 CXCL10。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠干扰素诱导T细胞趋化因子(ITAC;CXCL11)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠干扰素诱导T细胞趋化因子(ITAC;CXCL11)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat ITAC;CXCL11. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat ITAC;CXCL11 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.