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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
晚期糖基化终末产物受体(AGER),又称RAGE,是一种35千牛顿的免疫球蛋白超级家族的跨膜受体,主要包括糖蛋白,其糖类已经通过Maillard反应进行了非酶性修饰。RAGE基因位于6号染色体上的主要组织相容性复合体(MHC III类区)内,由11个外显子和10个内含子交错组成。该基因的总长度约为1400个碱基对(bp),包括启动子区域,该区域与PBX2基因部分重合。RAGE在体内有两种形式:一种是膜结合形式,称为mRAGE,另一种是可溶性形式,称为sRAGE。mRAGE有三个结构域,sRAGE只有细胞外结构域。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠晚期糖基化终末产物受体(RAGE;AGER)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠晚期糖基化终末产物受体(RAGE;AGER)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat RAGE;AGER. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat RAGE;AGER present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.