计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血红蛋白是红血球(红细胞)中的含铁运氧金属蛋白。血液中的血红蛋白将氧气从肺部带到身体的其他部位(即组织)。在那里,它释放氧气,允许有氧呼吸提供能量,为生物体的功能提供动力,这个过程称为新陈代谢。它也存在于红细胞及其原生系之外。其他含有血红蛋白的细胞包括黑质中的A9多巴胺能神经元、巨噬细胞、肺泡细胞、肺、视网膜色素上皮细胞、肝细胞、肾脏的间质细胞、子宫内膜细胞、宫颈细胞和阴道上皮细胞。在这些组织中,血红蛋白具有非载氧功能,是一种抗氧化剂和铁代谢的调节剂。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠游离血红蛋白(f-Hb)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠游离血红蛋白(f-Hb)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat f-Hb. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat f-Hb present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.