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白细胞介素 9(IL-9)是一种多能细胞因子(细胞信号分子),属于白细胞介素组。它由各种细胞,如肥大细胞、NKT 细胞、Th2、Th17、Treg、ILC2和Th9 细胞以不同数量产生。其中,Th9 细胞被认为是产生IL-9的主要CD4+T细胞。这种细胞因子刺激细胞增殖并防止细胞凋亡。它通过白细胞介素-9受体(IL9R)发挥作用,激活不同的信号转导者和激活者(STAT)蛋白,即STAT1、STAT3 和 STAT5,从而将这种细胞因子与各种生物学过程联系起来。编码这种细胞因子的基因已被确定为哮喘病的候选基因。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠白细胞介素9(IL-9)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠白细胞介素9(IL-9)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat IL-9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat IL-9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.