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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
粘蛋白2,也被称为MUC2,寡聚粘液凝胶形成,是由MUC2基因编码的蛋白质,是粘蛋白家族的成员。它覆盖肠道、气道和其他含有黏膜的器官的上皮,旨在为粘膜表面的颗粒和传染性病原体提供保护性的润滑屏障。它是结肠内外粘液层的主要成分,可能在从内粘液层排除细菌方面发挥作用。它在肠道中特别突出,它从上皮衬里的杯状细胞分泌到大肠的管腔中,在那里,粘蛋白2与少量相关的粘蛋白一起聚合成凝胶,其中80%的重量是寡糖侧链,作为粘蛋白的翻译后修饰添加。这种凝胶提供不溶性粘液屏障,用于保护肠上皮。它具有一个中心结构域,其中包含富含苏氨酸和脯氨酸的串联重复序列,在不同个体中在50至115个拷贝之间变化。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠粘蛋白2(MUC2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠粘蛋白2(MUC2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat MUC2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat MUC2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.