计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
糖蛋白130(又称gp130、IL6ST、IL6R-beta或CD130)是一种跨膜蛋白,是所有细胞因子受体类的创始成员。它构成了IL-6受体家族中I型细胞因子受体的一个亚单位。它通常被称为常见的gp130亚单位,对细胞因子参与后的信号转导很重要。与其他I型细胞因子受体一样,gp130拥有一个WSXWS氨基酸图案,以确保正确的蛋白质折叠和配体结合。它与Janus激酶相互作用,在受体与其配体相互作用后引起细胞内信号。从结构上看,gp130由五个纤连蛋白III型结构域和一个免疫球蛋白C2型(免疫球蛋白样)结构域在其细胞外部分组成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠糖蛋白130(gp130;CD130)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠糖蛋白130(gp130;CD130)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat gp130;CD130. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat gp130;CD130 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.