计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞色素c(cyt c)也称细胞色素复合体,是一种与线粒体内膜松散相连的小血蛋白,由CYCS基因编码。它属于细胞色素c蛋白家族,在细胞凋亡中起着主要作用。细胞色素c是高度水溶性的,与其他细胞色素不同,它是电子传输链的一个重要组成部分,它携带一个电子。它能够经历氧化和还原,因为它的铁原子在亚铁和铁的形式之间转换,但不与氧气结合。它在复合物III(辅酶Q-细胞c还原酶)和IV(细胞c氧化酶)之间转移电子。细胞色素c是一种跨物种的高度保守的蛋白质,在植物、动物和许多单细胞生物中发现。另外,它有一个初级结构,由大约100个氨基酸组成的链。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠细胞色素C(Cyt-C)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠细胞色素C(Cyt-C)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat Cyt-C. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat Cyt-C present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.