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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肝素结合性表皮生长因子(HB-EGF)是EGF家族中的一个成员,由HBEGF基因编码。HB-EGF样生长因子是作为一种膜锚定的有丝分裂和趋化糖蛋白合成的。由单核细胞和巨噬细胞产生的一种表皮生长因子,由于与肝素的亲和力,被称为HB-EGF。它已被证明在伤口愈合、心脏肥大以及心脏发育和功能中发挥作用。HB-EGF是一种87个氨基酸的糖蛋白,显示出高度调控的基因表达。它的跨膜形式是白喉毒素的独特受体,在细胞中发挥着并联信号的功能。HB-EGF的两种形式都参与正常的生理过程和病理过程,包括肿瘤进展和转移、器官增生和动脉粥样硬化疾病。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠肝素结合性表皮生长因子(HB-EGF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠肝素结合性表皮生长因子(HB-EGF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat HB-EGF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat HB-EGF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.