BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肾足蛋白(NPHN)是肾脏滤过屏障正常运作所必需的一种蛋白质。肾脏过滤屏障由裂隙内皮细胞、肾小球基底膜和上皮细胞的荚膜组成。它是一种跨膜蛋白，是裂隙隔膜的结构成分。它们以错综复杂的网状结构存在于荚膜细胞的顶端，传递强烈的负电荷，排斥蛋白质穿过鲍曼空间。它的基因缺陷，即NPHS1，与芬兰型先天性肾病综合征有关，导致大量蛋白质渗入尿液，即蛋白尿。它也是心血管发育所必需的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠肾足蛋白(NPHN)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠肾足蛋白(NPHN)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat NPHN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat NPHN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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