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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胎盘生长因子(PLGF)是一种蛋白质,是由PGF基因编码的。该基因是一个蛋白编码基因,是血管内皮生长因子(VEGF)家族的成员。PGF与血管生成有关。具体来说,PGF在滋养细胞的生长和分化中起作用,滋养层细胞,特别是绒毛外滋养层细胞,负责侵入子宫壁和母体螺旋动脉。PGF与VEGF竞争,以结合和激活VEGF R1/Flt-1。这通过使更多的VEGF可用于激活VEGF R2而导致血管生成增加。PGF还表现出与作为VEGF共同受体的Neuropilins-1和2的肝素依赖性结合。它可以抑制神经生长锥的崩溃,并诱导单核细胞产生包括血管内皮生长因子在内的炎症细胞因子。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠胎盘生长因子(PLGF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠胎盘生长因子(PLGF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat PLGF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat PLGF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.