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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胶质细胞系来源的神经营养因子(GDNF)是一种蛋白质,由GDNF基因编码。它于1991年被发现,是GDNF配体家族(GFL)的第一个成员。GDNF是一种小蛋白,能有效地促进多种类型的神经元的生存。它还负责将精原细胞确定为初级精母细胞,即它被RET接收,并通过与SCF形成梯度,将精原细胞分成两个细胞。GDNF的结构与TGFβ2相似,它有两个指状结构,与GFRα1受体相互作用。在原GDNF的分泌过程中发生的N-连接糖基化发生在其中一个指状结构的顶端。成熟的GDNF的C端在与Ret和GFRα1受体的结合中起着重要作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠胶质细胞系来源的神经营养因子(GDNF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠胶质细胞系来源的神经营养因子(GDNF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat GDNF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat GDNF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.