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脂解激活脂蛋白受体即脂肪分解刺激的脂蛋白受体 (LSR) 是 iota 毒素家族所有成员的宿主细胞受体,包括 CDT。LSR 是一种 I 型单通道跨膜蛋白,在蛋白质的细胞外部分具有一个 Ig 样 V 型结构域。该受体主要在肝脏中表达,但也在肠道和各种其他组织中表达。LSR 被鉴定为富含甘油三酯脂蛋白的肝脏受体,在将三细胞蛋白募集到三细胞接触中起作用,这些接触对上皮屏障的完整性很重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠脂解激活脂蛋白受体(LSR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠脂解激活脂蛋白受体(LSR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat LSR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat LSR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.