BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
芳烷基胺N-乙酰基转移酶(AANAT)是一个编码芳烃胺N-乙酰转移酶的基因。该基因所编码的蛋白属于乙酰转移酶超家族。它是合成褪黑激素的倒数第二种酶，控制脊椎动物松果体中褪黑激素的日/夜节律。褪黑激素对影响活动和睡眠的昼夜节律钟的功能至关重要。该酶受cAMP依赖性磷酸化的调节，促进其与14-3-3蛋白的相互作用，从而保护该酶免受蛋白体降解的影响。该基因可能导致许多遗传性疾病，如睡眠期延迟综合征。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠芳烷基胺N-乙酰基转移酶(AANAT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠芳烷基胺N-乙酰基转移酶(AANAT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat AANAT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat AANAT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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