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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
芳烃受体(又称 AhR、AHR、ahr、ahR 或二恶英受体)是一种蛋白质,是 由 AHR 基因编码的。芳烃受体是一种调控基因表达的转录因子。最初认为 它的功能主要是作为异生物化学品的传感器,同时也是代谢这些化学品的细 胞色素P450s等酶的调节器。这些异生物化学品中最引人注目的是芳香族(芳 基)碳氢化合物,该受体的名字就来源于此。最近,人们发现 AhR 被一些内 源性吲哚衍生物(如犬尿氨酸)激活(或失活)。除了调节代谢酶外,AhR 还具有调节免疫、干细胞维持和细胞分化的作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠芳香烃受体(AhR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠芳香烃受体(AhR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat AhR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat AhR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.