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葡萄糖转运蛋白4(GLUT4)，又称溶质载体家族2，促进葡萄糖转运体成员4，是一种蛋白质，由SLC2A4基因编码。GLUT4是受胰岛素调节的葡萄糖转运体，主要存在于脂肪组织和横纹肌（骨骼肌和心肌）。在细胞表面，GLUT4允许循环中的葡萄糖沿着浓度梯度扩散到肌肉和脂肪细胞。一旦进入细胞，葡萄糖会被肝脏的葡萄糖激酶和其他组织的己糖激酶迅速磷酸化，形成葡萄糖-6-磷酸，然后进入糖酵解或聚合成糖原。像所有的蛋白质一样，GLUT4的主序列中独特的氨基酸排列使它能够跨越质膜运输葡萄糖。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠葡萄糖转运蛋白4(GLUT4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠葡萄糖转运蛋白4(GLUT4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat GLUT4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat GLUT4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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