BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血小板膜糖蛋白Ⅳ(GP-Ⅳ)是一种胶原蛋白的糖蛋白受体，在血小板中表达。它是胶原、凝血酶、血管性假性血友病因子、纤维蛋白原和二磷酸腺苷等受体的成分，参与血小板黏附、聚集和活化反应，是生理性止血和病理性血栓形成的始动环节。研究显示，缺血性脑血管病的发生与血液的高凝状态有关。它作为介导血小板功能的关键效应分子，其表达水平明显影响着血小板的功能和活化状态，在止血中起着关键作用。它已被证明可与LYN相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠血小板膜糖蛋白Ⅳ(GP-Ⅳ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠血小板膜糖蛋白Ⅳ(GP-Ⅳ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat GP-Ⅳ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat GP-Ⅳ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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