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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
表皮生长因子受体(EGFR),是一种跨膜蛋白,是细胞外蛋白配体的表皮生长因子家族(EGF)的受体。它是ErbB受体家族家族的成员之一,ErbB家族是包含四种紧密联系蛋白的亚家族,这四种蛋白分别是受体酪氨酸激酶类:EGFR、HER2/c-neu、Her 3以及Her 4。它通过其特异性配体的结合被激活,包括表皮生长因子和转化生长因子α。在其生长因子配体激活后,EGFR经历从无活性单体形式到活性同源二聚体的转变。除了在配体结合后形成同源二聚体外,EGFR还可以与ErbB受体家族的另一个成员配对,例如ErbB2 / Her2 / neu,以产生活化的外源二聚体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠表皮生长因子受体(EGFR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠表皮生长因子受体(EGFR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat EGFR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat EGFR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.