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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
表皮调节素(EREG)是一种蛋白质,由 EREG 基因编码。它由 46 个氨基酸残基组成。它的二级结构中大约有 30%的β-片状结构。由于氢键的作用,一些残基形成了环状和转折。β-片状结构在表皮生长素中的比例取决于它们所处的领域和二级结构。它的聚合分子的公式重量为 5280.1g/mol,为多肽(L), 属于聚合物类型。它是表皮生长因子家族的一个成员,可作为表皮生长因子受体(EGFR)的配体,也可以作为 ERBB(v-erb-b2 肿瘤基因同源物)家族大多数成员的酪氨酸激酶受体的配体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠表皮调节素(EREG)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠表皮调节素(EREG)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat EREG. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat EREG present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.