BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
视黄醇结合蛋白4，也被称为RBP-4，是一种视黄醇（维生素A醇）的转运蛋白。RBP-4的分子量约为21kDa，由RBP-4基因编码。它主要（尽管不是唯一）在肝脏中合成，并在血液中循环，与视黄醇结合在一起，与转甲蛋白形成复合物。该蛋白属于脂联素家族，是血液中视黄醇（维生素A）的特定载体。它将视黄醇从肝脏储存中送到外周组织。在血浆中，RBP-视黄醇复合物与 transthyretin相互作用，防止其通过肾小球滤过而流失。维生素A的缺乏阻断了结合蛋白的翻译后分泌，导致对表皮细胞的输送和供应出现缺陷。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠视黄醇结合蛋白4(RBP-4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠视黄醇结合蛋白4(RBP-4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat RBP-4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat RBP-4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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