BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
角蛋白18，也被称为CK18，它是I型细胞角蛋白。它与其丝状体角蛋白8一起，可能是中间丝基因家族中最常见的产物。它们在身体的单层上皮组织中表达。该基因的突变与隐源性肝硬化有关。已经为该基因发现了编码相同蛋白质的两个转录物变体。它经常与角蛋白8和角蛋白19一起使用，以在血液循环肿瘤细胞计数测试中区分上皮细胞和造血细胞。当磷酸化时，在细丝重组中起作用。它参与将突变的CFTR递送至质膜。它与KRT8一起参与白细胞介素6 (IL-6)介导的屏障保护。它还参与肝细胞对凝血酶-抗凝血酶复合物的摄取。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠角蛋白18(CK18)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠角蛋白18(CK18)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat CK18. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat CK18 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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