BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Zonulin 是一种调节消化道壁细胞之间紧密连接的通透性的蛋白质。它是由 马里兰大学医学院的 Alessio Fasano 及其团队在 2000 年发现的。作为由霍 乱病原体霍乱弧菌分泌的紧密连接毒素的哺乳动物类似物，霍乱的典型症状 是大量的、水样的、衰弱的腹泻。与霍乱有关的细菌毒素之一，称为 "闭锁 区毒素"，能迅速和可逆地打开肠道细胞之间的紧密连接，暂时造成肠道泄漏。 zonulin 已与乳糜泻和 1 型糖尿病的发病机理有关。2 型糖尿病患者的 zonulin 增加。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠连蛋白(zonulin)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠连蛋白(zonulin)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat zonulin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat zonulin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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