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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
钙卫蛋白(CALP)是中性粒细胞产生的钙结合蛋白(约 36 kDa),占细胞总蛋白 质的 5%。具有组织或细胞特异性,可作为急性炎性细胞活化的标志物。它 由两条分子量为 14 kD 的重链和一条分子量为 8 kD 的轻链以共价键连接的 钙结合蛋白质异三聚体组成,具有耐热性和增强水解性的特性。1980 年由 Fagerhol 等首先从中性粒细胞中分离出来,命名为 L1 蛋白质。Dorin 和 Freemont 等进一步证实这种蛋白质具有 S-100 蛋白的结构特征,后来将此 种具有保护性、多功能、与钙结合的蛋白质命名为钙卫蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠钙卫蛋白(CALP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠钙卫蛋白(CALP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat CALP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat CALP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.