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钠尿肽受体 2/鸟苷酸环化酶 B(atrionatriuretic peptide receptor B),也 被称为 NPR2,是一种心房钠尿肽受体。它是由 NPR2 基因编码的。该基因 编码钠尿肽受体 2,是钠尿肽的两个整体膜受体之一。NPR1 和 NPR2 都含 有五个功能域:一个细胞外配体结合域,一个单一的膜跨区,以及细胞内的 一个蛋白激酶同源域,一个参与寡聚的螺旋铰链区,和一个羧基末端的鸟苷 酸环化酶催化域。该蛋白是 C 型钠尿肽(CNP)的主要受体,配体结合后表 现出大量的鸟苷酸环化酶活性。该基因的突变是引起尖锐湿疣马罗托型的原 因。NPR2 基因的突变可导致不相称的侏儒症和短肢。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠钠尿肽受体2(NPR2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠钠尿肽受体2(NPR2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat NPR2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat NPR2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.