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雄激素受体(AR)也被称为NR3C4(核受体亚家族3,C组,成员4),是一种核受体,通过在细胞质中结合任何一种雄性激素,包括睾酮和双氢睾酮而被激活,然后转移到细胞核。雄性激素受体与孕激素受体的关系最为密切,孕激素在较高剂量下可以阻断雄性激素受体。雄激素受体的主要功能是作为DNA结合的转录因子,调节基因的表达。它是前列腺癌的一个重要治疗目标。因此,已经开发了许多不同的抗雄激素,主要针对该蛋白的配体结合域。AR配体可以根据其结构(类固醇或非类固醇)或根据其激活或抑制转录的能力(激动剂或拮抗剂)进行分类。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠雄激素受体(AR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠雄激素受体(AR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat AR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat AR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.