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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
18kDa 阳离子抗菌肽(Cathelicidin 抗菌肽(CAMP))是一种多肽,主要储存在巨噬细胞和多形核白细胞(PMNs)的溶酶体中;CAMP 基因编码多肽前体 CAP-18(18 kDa),通过蛋白酶 3 介导的胞外裂解加工成活性形式LL-37 。 LL-37 是 在 身 体 内 发 现 的 唯 一 的 Cathelicidin 家 族 的 多 肽 。Cathelicidin 肽是双重性质的分子,称为两栖分子:分子的一端被水吸引,被脂肪和蛋白质排斥,另一端被脂肪和蛋白质吸引,被水排斥。这个家族的成员对病原体的反应是瓦解、破坏或刺破细胞膜。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠18kDa阳离子抗菌肽(CAMP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠18kDa阳离子抗菌肽(CAMP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CAMP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CAMP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.