BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
apelin 13(AP13)是一个由apelin基因编码的13个氨基酸的多肽，它产生一个预蛋白，经过加工后产生由36、17或13个氨基酸组成的生物活性肽（分别是apelin-36、apelin-17和apelin-13）。它是APJ受体的内源性配体，APJ受体是一种G蛋白偶联受体（EC50=370μM），在调节体液平衡、心血管功能和胰岛素敏感性方面起着重要作用。研究表明，Apelin-36能阻止免疫缺陷病毒进入细胞。然而，与Apelin-36不同，Apelin-13对HIV细胞进入的阻断作用很差。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠apelin 13(AP13)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠apelin 13(AP13)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse AP13. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse AP13 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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