计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ca2+ ATPase 是 P-ATPase 的一种形式,用于在肌肉收缩后转移钙。 质膜 Ca2+ ATPase(PMCA)是细胞质膜中的一种转运蛋白,用于从细胞中清除钙(Ca2+)。 它对调节细胞内 Ca2+ 的含量至关重要。 事实上,PMCA 参与清除所有真核细胞中的 Ca2+。 Ca2+的跨膜电化学梯度非常大,促使该离子进入细胞,但细胞必须保持低浓度的Ca2+,以发出适当的细胞信号;因此,细胞必须使用离子泵来清除Ca2+。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠Ca-ATP酶(Ca-ATPase)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠Ca-ATP酶(Ca-ATPase)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Ca-ATPase. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Ca-ATPase present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.