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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
C-C 趋化因子受体 3 型是一种蛋白质,由 CCR3 基因编码。CCR3 最近也被命名为 CD193(分化群 193)。该基因所编码的蛋白是 C-C 型趋化因子的受体。它属于 G 蛋白偶联受体的第 1 家族。该受体与多种趋化因子结合并产生反应,包括 Eotaxin(CCL11)、Eotaxin-3(CCL26)、MCP-3(CCL7)、 MCP-4(CCL13)和 RANTES(CCL5)。它在嗜酸性粒细胞和嗜碱性粒细胞中高度表达,并在 TH1 和 TH2 细胞以及气道上皮细胞中检测到。这种受体可能有助于嗜酸性粒细胞和其他炎症细胞在过敏性气道中的积累和激活, 也可能在寄生虫感染的部位。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠CC趋化因子受体3(CCR3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠CC趋化因子受体3(CCR3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CCR3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CCR3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.