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Fractalkine(FKN)也被称为CX3C趋化因子配体1,是一种由CX3CL1基因编码的蛋白质。FKN是一个有373个氨基酸的大型细胞因子蛋白,它包含多个结构域,是CX3C趋化因子家族中唯一已知的成员。CX3CL1的多肽结构与其他趋化因子的典型结构不同。例如,特征性的N端半胱氨酸的间距不同;在CX3CL1中,最初的一对半胱氨酸之间有三个氨基酸,而在CC趋化因子中没有,在CXC趋化因子中只有一个氨基酸的间隔。CX3CL1是一个长的蛋白质,有一个延长的粘液蛋白样柄和一个趋化因子结构域在上面。粘液蛋白样柄使它能与某些细胞的表面结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠CX3C趋化因子;fractalkine(FKN;CX3CL1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠CX3C趋化因子;fractalkine(FKN;CX3CL1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse FKN;CX3CL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse FKN;CX3CL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.