计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
趋化因子配体 14(CXCL14)是一种属于 CXC 趋化因子家族的小细胞因子,也被称为 BRAK(代表乳腺和肾脏表达的趋化因子)。成熟的 CXCL14 具有CXC 趋化因子亚家族的许多保守特征,但也有一些不同之处,如 N 端较短,在其第三和第四个半胱氨酸之间的区域有五个额外的氨基酸。CXCL14 在许多正常组织中呈高水平表达,其细胞来源被认为是成纤维细胞。这种趋化因子对单核细胞有趋化作用,在有一种叫做前列腺素-E2(PGE2)的炎症介质存在时能激活这些细胞。它也是树突状细胞的强效化合剂和激活剂,与这些细胞的归巢有关,并能刺激活化的 NK 细胞的迁移。CXCL14 还能抑制血管生成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠CXC趋化因子配体14(CXCL14)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠CXC趋化因子配体14(CXCL14)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CXCL14. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CXCL14 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.