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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Runt 相关转录因子 3,也被称为 RUNX3,是由 RUNX3 基因编码的蛋白质。该基因编码含有 runt 结构域的转录因子家族的成员。这种蛋白质的异二聚体和一个β亚基形成一个复合物,与许多增强子和启动子中的核心 DNA 序列5'-YGYGGT-3'结合,可以激活或抑制转录。它还与其他转录因子相互作用。它起到肿瘤抑制因子的作用,并且该基因在癌症中经常被删除或转录沉默。已经为该基因发现了编码不同同种型的多个转录变体。在黑素细胞中,该基因的表达可能受 MITF 调节。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠Runt相关转录因子3(RUNX3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠Runt相关转录因子3(RUNX3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse RUNX3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse RUNX3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.