计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β2微球蛋白(BMG;β2-MG)由B2M基因编码。它位于细胞表面的α3链旁边。与α3不同,β2没有跨膜区。在β2的正上方(即离细胞更远)是α1链,它本身就在α2的旁边。β2微球蛋白不仅与MHC I类分子的α链结合,而且还与I类分子如CD1、MR1、和Qa-1(异体抗原的一种形式)结合。它的另一个功能是与HFE蛋白相关联,共同调节肝脏中血球素的表达,血球素针对肠细胞基底膜和巨噬细胞细胞膜上的铁转运体ferroportin进行降解,分别导致从食物中摄取的铁减少和从MPS(单核吞噬细胞系统)中回收的红细胞释放的铁减少。这一功能的丧失会导致铁过量和血色素沉着症。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠β2微球蛋白(BMG;β2-MG)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠β2微球蛋白(BMG;β2-MG)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse BMG;β2-MG. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse BMG;β2-MG present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.