计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙醇脱氢酶(Alcohol dehydrogenase),简称 ADH,大量存在于动物肝 脏、植物及微生物细胞中,是一种含锌金属酶,具有广泛的底物特异性。它 是一个质量为 80kDa 的二聚体,包括一组同工酶,这些同工酶都能够将乙醇 转化为乙醛。在哺乳动物中,这是一个涉及辅酶烟酰胺腺嘌呤二核苷酸 (NAD+)的氧化还原反应。乙醇脱氢酶负责催化氧化伯醇和二级醇成为醛 和酮,另外也可以影响它们的逆反应。在哺乳动物体内,乙醇脱氢酶与乙醛脱 氢酶(ALDH)构成了乙醇脱氢酶系,参与体内乙醇代谢,是动物体内重要的代 谢酶。作为生物体内主要短链醇代谢的关键酶,它在很多生理过程中起着重 要作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠乙醇脱氢酶(ADH)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠乙醇脱氢酶(ADH)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ADH. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ADH present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.