BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管生成素2是一种蛋白质，由ANGPT2基因编码的。血管生成素是在血管发育和血管生成中具有重要作用的蛋白质。所有血管生成素都以类似的亲和力与内皮细胞特异性的酪氨酸蛋白激酶受体结合。血管生成素2是ANGPT1和TIE2的天然拮抗剂，只在血管重塑的部位表达。血管生成素-2 产生并储存在内皮细胞的 Weibel-Palade 小体中，并作为 TEK 酪氨酸激酶拮抗剂。结果，促进了内皮活化、不稳定和炎症的促进。它在血管生成过程中的作用取决于 Vegf-a 的存在。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠促血管生成素2(ANG-2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠促血管生成素2(ANG-2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ANG-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ANG-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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