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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
单胺氧化酶(MAO)是一个催化单胺氧化的酶家族,利用氧来夹掉它们的胺基。它们被发现结合在身体大多数类型的细胞的线粒体外膜上。第一个这样的酶是在1928年由Mary Bernheim在肝脏中发现的,并被命名为酪胺氧化酶。MAO属于含黄素胺氧化还原酶的蛋白质家族。它在分解食物中摄取的单胺类物质方面非常重要,同时也起到灭活单胺类神经递质的作用。单胺氧化酶催化单胺的氧化脱氨反应。在反应的第一部分,辅因子 FAD 氧化底物,产生相应的亚胺,该亚胺将辅因子转化为其还原形式 FADH2。然后将亚胺非酶水解成相应的酮(或醛)和氨。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠单胺氧化酶(MAO)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠单胺氧化酶(MAO)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MAO. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MAO present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.