BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
卵泡抑素样蛋白1(FSTL1)是一种蛋白质，由FSTL1基因编码。该基因编码是一种与BMP-4结合蛋白follistatin相似的蛋白质。它与BMP-4和TGF-β1结合，但不与Activin A结合。它包含一个FS模块（一个含有10个保守半胱氨酸残基的follistatin样序列）、一个Kazal型丝氨酸蛋白酶抑制域、2个EF手域和一个Von Willebrand因子C型域。它在发育中具有一定的作用，如肺部发育，输尿管发育，中枢神经系统发育和骨骼发育等。它能上调关节炎病理中重要的促炎症介质，血清中FSTL1的水平与关节炎的严重程度相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠卵泡抑素样蛋白1(FSTL1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠卵泡抑素样蛋白1(FSTL1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse FSTL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse FSTL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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