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肿瘤坏死因子可溶性受体Ⅰ(sTNFR1)是一种具有生物学活性的分泌性蛋白。 TNF 受体有 TNFR1(55KD)和 TNFR2 (75KD)2 种，均为糖蛋白，其中 TNFR1 得了大部分 TNF 的生物学功能，如抗病毒、诱导凋亡、活化 NFkB、成纤维 细胞增殖等。TNFR 分为膜结合型(mTNFR)和可溶型(sTNFR)2 种类型。可溶 型受体为膜结合型受体胞外区在 TNF 转换酶(TAGE)的作用下从细胞膜上解 离下来，游离在血液中，仍可与 TNF 结合，但是无法介导 TNF 的信号传导。 因此 sTNFR 能够竞争性地与 TNF 分子结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠可溶性肿瘤坏死因子受体1(sTNFR1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠可溶性肿瘤坏死因子受体1(sTNFR1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse sTNFR1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse sTNFR1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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