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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
基质金属蛋白酶-1(MMP-1),也称为间质胶原酶和成纤维细胞胶原酶,是由MMP1基因编码的酶。该基因是位于染色体11q22.3的MMP基因簇的一部分。它是第一种脊椎动物胶原酶,既可以纯化为蛋白质均质,也可以克隆为cDNA。它的估计分子量为54kDa。它作为酶原合成,其中 N 端残基通过蛋白水解被去除,并与其他MMP共享一个催化结构域和一个与血红素结合素序列相似的羧基末端结构域。它被认为是一种多功能分子,因为它不仅参与细胞外空间中胶原纤维的更新,而且参与许多非基质底物和细胞表面分子的裂解,这表明它在调节细胞行为。此外,大量证据表明它在多种生理过程中发挥重要作用,例如发育、组织形态发生和伤口修复。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠基质金属蛋白酶1(MMP-1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠基质金属蛋白酶1(MMP-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MMP-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MMP-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.